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KMID : 0613820070170030420
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Journal of Life Science
2007 Volume.17 No. 3 p.420 ~ p.426
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Effects of dietary polyphenol (-)-epigallocatechin-3-gallate on the differentiation of mouse C2C12 myoblasts
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Kim Hye-Jin
Lee Won-Jun
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Abstract
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In the present investigation, we studied the modulating effects of (-)-epigallocatechin-3-gallate(EGCG) on the differentiation of mouse C2C12 myoblasts. We found that the strong inhibitory effect of EGCG on DNA methyltransferase-mediated DNA methylation induced transdifferentiation of C2C12 myoblasts into smooth muscle cells demonstrated by both morphological changes and immunofluorescent staining. C2C12 myoblasts treated with EGCG for 4 days expressed smooth muscle α-actin protein. Real-time PCR data revealed that smooth muscle α-actin mRNA was induced by EGCG treated C2C12 myoblasts in a concentration-dependent manner. Smooth muscle α-actin mRNA concentration increased 330% and 490% after 2 and 3 days of 50 μM of EGCG treatment. The expression of another smooth muscle marker, transgelin, mRNA was also increased up to 9-fold by 4 days of EGCG treatment compared with control in a concentration-dependent manner. These results suggested that C2C12 enables to transdifferentiate into smooth muscle when gene expression patterns are changed by the inhibition of DNA methylation induced by EGCG. In conclusion, transdifferentiation of C2C12 myoblasts into smooth muscle is resulted from the modulating effects of EGCG on DNA methylation which subsequently results in changing the expression pattern of several genes playing a critical role in the differentiation of C2C12 myoblasts.
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KEYWORD
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DNA methylation, smooth muscle, differentiation, EGCG, C2C12 myoblasts
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